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Dionex BorateTrap 硼酸鹽捕獲色譜柱

更新時間:2019-11-12

簡要描述:

Dionex BorateTrap 硼酸鹽捕獲色譜柱
當放置洗脫液泵和注射閥,陷阱上的碳水化合物分析物的效率或保留時間沒有影響之間。

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Dionex  BorateTrap 硼酸鹽捕獲色譜柱

對于使用HPAE-PAD檢測IC碳水化合物分析中*的性能,使用的Thermo Scientific戴安BorateTrap在線捕獲柱除去硼酸鹽污染。當放置洗脫液泵和注射閥,陷阱上的碳水化合物分析物的效率或保留時間沒有影響之間。的戴安BorateTrap柱消除了甘露糖,果糖,以及從硼酸污染引起糖醇峰拖尾。

 

 

Dionex  BorateTrap 硼酸鹽捕獲色譜柱

訂貨信息:

Dionex  BorateTrap 硼酸鹽捕獲柱

Borate   Trap Column              047078

 

The BorateTrap is an in-line trap column, and is recommended for optimal performance during carbohydrate analysis.

  • In-line trap column removes borate contamination for HPAE-PAD.

  • Eliminates peak tailing for mannose, fructose, and sugar alcohols resulting from borate contamination. 

Borate contamination in eluents can cause a significant loss of peak efficiency, especially for mannose, fructose, and reduced monosaccharides. If borate is present in the eluent, it binds both to the anion-exchange column and to the carbohydrate analytes. The carbohydrate-borate complex is less efficiently eluted from the anion-exchanger than is the carbohydrate, resulting in peak tailing, particularly where vicinal cis hydroxyl groups are present, such as for mannose and sugar alcohols. The BorateTrap eliminates peak tailing for mannose, fructose, and reduced monosaccharides, resulting from contamination of the eluent from borate. Therefore, the BorateTrap is highly recommended for optimal performance during carbohydrate analysis.

The BorateTrap is a 4 × 50 mm column placed between the eluent pump and the injection valve to remove borate contamination from eluents used for HPAE-PAD. Because it is placed before the injection valve, it has no effect on the efficiencies or retention times of the carbohydrate analytes. This column is packed with a 20-μm high-capacity resin that has high selectivity for borate. The resin used in the BorateTrap is functionalized with a polyol capable of complexing borate in the presence of hydroxide. Borate is a known contaminant in laboratory water supplies. In chromatography, borate contamination of HPLC eluents may be a result of degrading deionized water systems or as leachate from borosilicate glassware.

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